Nucleotide binding to creatine kinase: an isothermal titration microcalorimetry study
نویسندگان
چکیده
منابع مشابه
New insights into chitosan-DNA interactions using isothermal titration microcalorimetry.
The interaction of chitosan with plasmid DNA was investigated as a function of pH, buffer composition, degree of deacetylation (DDA), and molecular weight (M(n)) of chitosan, using isothermal titration microcalorimetry (ITC). The Single Set of Identical Sites model was used to obtain the enthalpy of interaction, the binding constant, and the stoichiometry of binding. The chitosan-DNA interactio...
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The main calorimetric principles used in isothermal microcalorimetry are briefly discussed. Different chemical calibration and test reactions are discussed, with a focus on reactions suitable for ambient conditions: reactions initiated by mixing of liquids (including titration microcalorimetry), dissolution of solid compounds and of slightly soluble gases, a photochemical process, and thermal p...
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The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for and , and also their molar enthalpies of binding (KJ/mol for and KJ/mo...
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Iron deposition within the iron storage protein ferritin involves a complex series of events consisting of Fe(2+) binding, transport, and oxidation at ferroxidase sites and mineralization of a hydrous ferric oxide core, the storage form of iron. In the present study, we have examined the thermodynamic properties of Fe(2+) binding to recombinant human H-chain apoferritin (HuHF) by isothermal tit...
متن کاملAn interlaboratory study of creatine kinase and creatine kinase isoenzymes.
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire regarding methodology. The specimens were prepared from an homogenate of human myocardium that had been centrifuged, dialyzed, and added to a matrix...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1999
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(99)01431-3